| 質粒名稱: | pET-15b PAI-RBP |
|---|---|
| 出品公司: | Novagen |
| 目錄編號: | 45390 |
| 存儲實驗室: | Thomas Gelehrter |
|
載體骨架基本信息 |
|
| 載體名稱: | pET15b |
| 載體出品公司: | Novagen |
| 空載體大小: | 5700 bp |
| 完整質粒大小: | 6900 bp |
| 載體類型: | 細菌細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | PAI-RBP / DKFZp564M2423 |
| 物種來源: | H. sapiens (human) |
| 插入大小: | 1170 bp |
| 基因突變: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank編號 | AL080119 |
| 啟動子: | CMV |
| 標簽/融合蛋白: | His (N terminal on insert) |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | ANde I(未破壞) |
| 3’克隆位點: | BamH I(未破壞) |
| 5’測序引物 | 5'-TAATACGACTCACTATAGGG-3' Novagen |
| 3’測序引物 | 5'-GCTAGTTATTGCTCAGCGG-3' Novagen |
|
文獻引用方法 |
|
| 材料和方法部分: | pET-15b PAI-RBP was a gift from Thomas Gelehrter (Addgene plasmid # 45390 ; //n2t.net/addgene:45390 ; RRID:Addgene_45390) |
| 參考文獻部分: | Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the Type-1 plasminogen activator inhibitor mRNA. Heaton JH, Dlakic WM, Dlakic M, Gelehrter TD. J Biol Chem. 2001 Feb 2;276(5):3341-7. Epub 2000 Sep 22. 10.1074/jbc.M006538200 PubMed 11001948 |
買家導航
pET-15b PAI-RBP載體質粒基本信息
pET-15b PAI-RBP質粒圖譜載體圖譜和pET-15b PAI-RBP載體序列質粒序列多克隆位點信息
